Create a workflow diagram of Illumina Next-Generation Sequencing: (1) genomic DNA extraction and fragmentation by sonication, (2) end repair, A-tailing, and adapter ligation, (3) size selection and PCR amplification, (4) cluster generation on flow cell by bridge amplification, (5) sequencing-by-synthesis with fluorescent reversible terminators, (6) base calling and FASTQ generation, (7) bioinformatics pipeline (alignment, variant calling, annotation). Show each step with equipment schematics.
